Evaluation of eight protocols for genomic DNA extraction of Hypostomus commersoni Valenciennes, 1836 (Loricariidae: Siluriformes)

Abstract The principle and the techniques applied in DNA extraction play a pivotal role obtention of purified genetic material. present study investigates efficiency eight protocols Hypostomus commersoni, an essential component South American freshwater ichthyofauna. quality samples was assessed through spectrophotometry, gel electrophoresis, PCR-RAPD markers amplification. influenced both by method target-tissue choice. Higher concentrations yield were obtained from ocular tissue, with positive spectrum incubation lysis buffer for up to 36 hours after sample collection, using fresh tissues presence high concentration Proteinase K (20 mg.ml-1). In these conditions, successfully amplified. To date, there is no record description parameters analyzed this work, neither RAPD species H. commersoni.